Molecular Characterization of Circulating Duck Viral Enteritis in Egypt During 2012-2013

Abstract

2 Abstract: Isolation trials were made on Duck Viral Enteritis (DVE) collected from natural outbreak in 2012 and 2013 from different breeds, age and location. The collected samples were processed for isolation and identification of DVE. Characterizations and pathogenesis of the isolates were studied by inoculation in specific pathogen free; embryonated Muscovy eggs and one-day old Muscovy ducklings, then keep for observation for clinical sings and mortality. Liver, eosophagus, small intestine and kidney collected from field samples, inoculated embryos and ducklings are examined histopathologically. Supernatant from tissue suspension of original samples was examined with PCR. Impression smears from organs of both field cases and inoculated ducklings examined with Indirect Fluorescent Antibody Test (IFAT). Many basophilic and esinophilic intranuclear inclusion bodies were observed hisotopathologically in 2 samples of 4, in livers of SPF, embryonated Muscovy eggs, ducklings one-day old. Fluorescent green light colors were seen in the same samples and in Muscovy ducklings' liver and eosophagus. PCR results were agreed with isolation on SPF, embryonated Muscovy eggs, one-day old Muscovy ducklings and Indirect Fluorescent Antibody Test (IFAT).