Evaluation of Antioxidant Activity of Clitoria ternatea and Alternanthera sessilis Plant Extracts Using Model System for Yeast Cells

Abstract

2 Abstract: To evaluate the antioxidant effects and apoptotic study of the leaves of Clitoria ternatea and Alternanthra sessilis by using the yeast cell. The yeast cells were isolated from the sugar factory effluents and isolated the yeast cell DNA. The leaves extract from different solvents were tested for their scavenging activity against the stable free radical DPPH (2, 2'-diphenyl-1-picryl hydrazyl) in dot plot rapid screening assay method and quantified using a spectrophotometric assay method. Oxidative damage was induced in vitro by treating yeast DNA and analyse the effects of the leaf extracts. Genomic DNA samples were isolated from YBD broth culture. DPPH scavenging activity was highly elicited by the methanol extract of C. ternatea and aqueous extract of A. sessilis. The C. ternatea and A. sessilis leaf extracts treatment effectively decreased the extent of DNA damage. C. ternatea and A. sessilis leaves have the potential antioxidant activity. Thus validating it is a source of valuable antioxidant drugs.