A COMPARATIVE EVALUATION OF ANTICANCER ACTIVITIES OF FLAVONOIDS ISOLATED FROM MIMOSA PUDICA, ALOE VERA AND PHYLLANTHUS NIRURI AGAINST HUMAN BREAST CARCINOMA CELL LINE (MCF-7) USING MTT ASSAY

Abstract

Objective: The present study was designed to determine the comparative anticancer activities of flavonoids isolated from Aloe vera, Mimosa pudica and Phyllanthus niruri against human breast carcinoma cell line (MCF-7) using MTT assay. Methods: MTT-based cytotoxicity activity study against human breast carcinoma cell line (MCF-7) was conducted to evaluate the potent activity of flavonoids isolated from Aloe vera, Mimosa pudica and Phyllanthus niruri. Thin Layer Chromatography (TLC) and Fourier Transform Infra-Red (FTIR) spectra were recorded which confirmed the presence of flavonoids in the methanolic extracts. Results: Flavonoids isolated from Aloe vera, Mimosa pudica and Phyllanthus niruri showed cytotoxicity activity against human breast carcinoma cell line (MCF-7) and the inhibitory concentration at 50% growth (IC50) was found to be, Mimosa pudica (IC50= 35.52±0.50µg/ml), Aloe vera (IC50= 54.97±0.36µg/ml) and Phyllanthus niruri (IC50= 84.88±0.87µg/ml). Conclusion: The results indicated the cytotoxicity activity of all the three flavonoids isolated. Therefore, they can be effectively employed in anticancer treatment. Cytotoxic study suggested that flavonoid from Mimosa pudica has the maximum cytotoxic effect than flavonoid from Aloe vera and Phyllanthus niruri against MCF-7, Human breast cancer cell line (Mimosa pudica >Aloe vera >Phyllanthus niruri).