Evaluation of Antibacterial Activity and Phytochemical Screening of Medicago sativa Leaves

Abstract

The in vitro antibacterial activity of various solvent extracts of Indian traditional medicinal plant Medicago sativa Leaves against clinical pathogens of human origin was evaluated. The antimicrobial activity of different solvents crude extract of plant used in traditional Indian medicine was tested by disc diffusion and turbidity assay method against three bacterial pathogens. The present screening result demonstrated that the Indian traditional medicinal plant Medicago sativa leaves methanol extract has potent antibacterial activity. The studied plant may be new source for novel antibacterial compound discovery for treating drugs resistant human pathogens as the results revealed that extracts exhibited a significant broadspectrum antibacterial activity against both Gram-positive and Gram-negative bacteria.