Evaluation of Antimicrobial and Anticancer activities of Methanol Extract of in vivo and in vitro grown Bauhinia variegata L.

Abstract

The present investigation deals with the biological activities of the extracts of the medicinal plant Bauhinia variegata L.[BV], generated through in vivo and in vitro processes for their antibacterial and anticancer activities. Nodal explants of BV when placed on MS medium fortified with 6-Benzyl amino purine (BAP) at 5µg/ml resulted into multiple shoots. These shoots, on transfer developed bunch of roots in presence of Indole-3-butyric acid (IBA) at 2-4 µg/ml. The methanol extracts of such regenerated in vitro plants along with that of natural in vivo garden plants on comparison were found to be more effective against gram positive bacteria when compared to gram negative bacteria. But the screening of the in vitro cytotoxicity on EAC mouse cell lines responded almost with the same degree of inhibition for the ethanol extract, derived from both in vivo and in vitro sources.