High Frequency Shoot Regeneration from Hypocotyl of Canola (Brassica napus L.) cv. Dunkled

Abstract

Hypocotyl explants of Brassica napus L. cv. Dunkled were used to investigate the effects of various plant growth regulators and silver nitrate on morphogenesis in vitro. Two types of explants, such as upper and lower portion of hypocotyl were examined. A high frequency of shoot regeneration (96 %) was achieved on MS supplemented with growth regulators. Shoots regenerated earlier (within three weeks) from segments taken from the upper portion of the hypocotyl than those from lower portion (after three weeks), although shoot regeneration frequency was similar in both the cases. Exclusion of silver nitrate from the medium drastically reduced the regeneration potential (50 %). Multiple shoot regeneration was obtained from the periodic subculture of the explants in the same medium. In vitro elongated shoots were rooted with a 100 % success by treating them with half-strength MS supplemented with 0.250 mg/l IBA and 0.125 mg/l IAA. Rooted plantlets were successfully established into the soil.