Effectsof Camellia sinensis Ethanolic Extract on Histometric and Histopathological Healing Process of Burn Wound in Rat

Abstract

5 Abstract: Green tea (Camellia sinensis) that were used as an ancient herbal drug in Asia, with having anti-oxidant and anti-inflammatory properties, may can improve wound healing process, thereforethe present study were design to determinate the effect of green tea ethanolicextract on burn wound healing process in rat. For this experimental study, 36 healthy male Wistar rats were randomly selected into three groupsof A, B and Cwhich, respectively treated with, Vaseline + 0.6% green tea extract, Vaseline andnormal salinefor a 21 days period. Burn Wounds' areas were measured and calculated by caliper every other day and pathologic samples were taken at 3 , 12 and 21 day for histopathological examinations. In the end, all data were analyzed by SPSS rd th st 16 using survival analysis (Breslow test), repeated measured ANOVA, one-way ANOVA and Mann-Whitney. P<0.05 was considered as statistically significant.Regarding Breslow test, the mean healing duration in group A with18 days was shorter in compared to group B with 20.33 days, (P=0.023). In according to Mann-Whitney test, comparing groups A and C during the whole study, indicated no statistically significant differences in histopathologicalscoresof epithelial regeneration and angiogenesis except inflammatory cells. Green tea extract could help wound healing process, probably effective on burn wounds healing.