Comparative Molecular and Conventional Detection Methods of Babesia equi (B. Equi) in Egyptian Equine

Irene Sameh Gamil, Manal M. Hussein

2011 · 18 citations · 2 references

Concepts

Abstract

2 Abstract: Theileria equi is a tick-borne hemoprotozoan parasite and one of the causative agents of equin e piroplasmosis. Blood is collected from a total of 100 horses. Three diagnostic methods: microscopical examination, enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), were compared in detecting T. equi. The results showed that the prevalence rate of T. equi in examined horses was 18%, 30% and 26% by using microscopic examination, ELISA and PCR, respectively.Clinical, hematological and biochemical studies were evaluated in examined horses. Significant decrease in hematological parameters was observed along with an increase in some biochemical parameters. The results of PCR assay showed its importance in the diagnosis of the carrier infected cases more than with the conventional techniques. The microscopic examination was used only in acute cases of the disease.

References

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