Antimicrobial Activity of Leaf Extracts of Murraya Koenigii against Aerobic Bacteria Associated with Bovine Mastitis

Abstract

The susceptibilities of some of the common aerobic bacterial isolates from 20 milk samples collected from different cows with udder inflammation were subjected to hexane and methanolic leaf extracts of Murraya koenigii was determined by the well diffusion method. The minimum inhibitory concentration (MIC) of test extracts that gave inhibition were determined using the tube dilution method. The phytochemicals present in the various leaf extracts were also qualitatively assayed using conventional techniques. The methanolic extract of leaves of Murraya koenigii inhibited Staphylococcus aureus , Staphylococcus epidermidis, Streptococcus uberis, Pseudomonas aeruginosa, Escherichia coli, Corynebacterium gravis and Bacillus cereus . The hexane extract inhibited all microorganisms except Staphylococcus epidermidis , Streptococcus uberis and Bacillus cereus . The MIC values of the different methanolic extracts of leaves were found to vary greatly, and ranged from 8.25 mg/ml to 30mg/ml.