In vitro Regeneration in Lentil (Lens culinaris Medik.)

Abstract

Four varieties of lentil (Lens culinaris Medik.), namely BM-1, BM-2, BM-3 and BM-4 of microsperma type were used in in vitro regeneration. Among various explants cotyledonary nodes from BM-2 and BM-4 were found to be the best for multiple shoot formation; decapitated embryos being the second best. Best multiple shoot regeneration was achieved on MS medium supplemented with 0.5 mg/l BAP, 0.5 mg/l Kn, 0.1 mg/GA3 and 5.5 mg/l tyrosine. Moderate success in root development was achieved on MS supplemented with 25 mg/l IBA.