Optics Express · 2015 · 45 citations · 16 references
EngineeringBiomedical EngineeringBiophysicsBiomedical OpticsOpticsPhotonicsFluorescence ImagingFluorescence MicroscopyLight MicroscopyOptical ImagingPlasmonicsSuper-resolution MicroscopyBiological ImagingDynamic ImagingInterference PatternsMicroscopyMicroscopy ImagingOptical VorticesStructured Illumination MicroscopySuper-resolution Imaging
We demonstrate an all-optical plasmonic structured illumination microscopy (PSIM) technique. A set of plasmonic standing-wave patterns is excited by amplitude-modified optical vortices (OVs), which have fractional topological charges for precise phase shift of {-2π/3, 0, 2π/3}. A specially designed optical aperture is introduced to modify the OVs in order to improve the uniformity of interference patterns. The imaging results of fluorescent beads reveal a sub-100nm resolving capability in aqueous environment. This PSIM technique as a structure-free, wide-field and super-resolved imaging technique is of great potential for low-cost biological dynamic imaging applications.
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Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement
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