Zenodo (CERN European Organization for Nuclear Research) · 2014 · 16 citations · 0 references
Veterinary MedicineImmunologyInfectious DiseasesDiagnostic ParasitologyNanobiotechnologyParasitologyAntigen Detection AssaysHelminth DiagnosticsImmunomagnetic SeparationNanomaterial-based BiosensorsNanoparticle-based ImmunotechnologyParasitic Disease DiagnosisImmunomagnetic PurificationLatex Agglutination AssaysLatex Agglutination TestsMagnetic ImmunosensorsMagnetic Nanoparticle BiosensorsParasitic Antigen DetectionParasitic SerologySerological Diagnosis Of Parasitic Infections
The objective of the present study was to evaluate the novel nanomagnetic beads based–latex agglutination assay (NMB-LAT) as a simple test for diagnosis of S. <em>haematobium</em> as well as standardize the novel nanomagnetic beads based –ELISA (NMB-ELISA). According to urine examination this study included 85 S. <em>haematobium</em> infected patients, 30 other parasites infected patients and 25 negative control samples. The sensitivity of novel NMB-LAT was 82.4% versus 96.5% and 88.2% for NMB-ELISA and currently used sandwich ELISA respectively. The specificity of NMB-LAT was 83.6% versus 96.3% and 87.3% for NMB-ELISA and currently used sandwich ELISA respectively. In conclusion, the novel NMB-ELISA is a valuable applicable diagnostic technique for diagnosis of human schistosomiasis <em>haematobium</em>. The novel NMB-ELISA assay is a suitable applicable diagnostic method in field survey especially when followed by ELISA as a confirmatory test in query false negative results. Trials are required to increase the sensitivity and specificity of NMB-ELISA assay.