Life SciencesBiochemistryFood ScienceFood ChemistryCarbohydrate AbsorptionEnzyme-linked Immunosorbent AssayGut MicrobiotaIntestinal AbsorptionIntestinal Barrier FunctionBrush Border DisaccharidasesDisaccharidase ActivityDisaccharide Digestion
SummaryThe method for disaccharidase activity assay in peroral biopsies of the human small-intestinal mucosa is described in detail. The specificity of the enzymes and the clinical conditions affecting the intestinal disaccharidase activity are discussed.