Molecular and Cellular Biology · 1988 · 39 citations · 31 references
Life SciencesGeneticsMolecular BiologyGene ExpressionTranscriptionTranscriptional RegulationCore Promoter ElementsGene Expression RegulationGene Regulatory ElementsPromoter ArchitecturePromoter RegulationTranscription Start SitesGene Promoter RegulationGenomic Dna AnalysisMalic Enzyme RegulationPromoter Activity AnalysisPromoter CharacterizationPromoter Sequence DeterminantsPromoter Sequence MotifsPromoters
We have characterized sequences of genomic DNA 5' to the coding region of the rat malic enzyme gene. This sequence possesses neither TATA nor CCAAT sequences in their usual positions but is rich in GC residues. Sequences similar to those found in the regulatory regions of other genes are discussed. Deletion analyses have revealed that sequences +1 to -41 are sufficient to initiate expression, although inclusion of information up to -177 is necessary for maximal promoter activity.
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