Resistance-related metabolites in wheat against <i>Fusarium graminearum</i> and the virulence factor deoxynivalenol (DON)

Abstract

Inoculation with the virulence factor deoxynivalenol (DON) can induce disease symptoms in wheat ( Triticum aestivum L.) spikelets, even though it is not needed for the initial invasion by Fusarium graminearum Schwabe, thus the mechanism of plant defense against both the pathogen and DON, was investigated. Wheat cultivars that are resistant (‘Sumai3’) or susceptible (‘Roblin’) to fusarium head blight (FHB) were inoculated with F. graminearum, DON, or water. Inoculated spikelets were harvested 48 h after inoculation, the metabolites were extracted in methanol–water and chloroform, then derivatized and analyzed by gas chromatography – mass spectrometry. The metabolite peaks were deconvoluted and identified by manually matching the mass spectra with those in the NIST and GMD libraries. The peaks were aligned, and abundances were measured. A total of 117 metabolites were tentatively identified, including several antimicrobial metabolites and signal molecules or their precursors. Out of these 117 metabolites, 15 and 18 were identified as possible resistance-related (RR) metabolites, following F. graminearum (RRIF) and DON (RRID) inoculations, respectively, with 4 metabolites common to both. Canonical discriminant analysis of marginally significant metabolites (105) identified those with constitutive and induced resistance functions. The metabolites with high canonical loading to the canonical vectors were used to explain these functions. The putative roles of these RR metabolites in plant defense, their metabolic pathways, and their potential application for screening of wheat breeding lines for resistance to FHB are discussed.