Cascade Reactions in Multicompartmentalized Polymersomes

Abstract

Abstract Enzyme‐filled polystyrene‐ b ‐poly(3‐(isocyano‐ L ‐alanyl‐aminoethyl)thiophene) (PS‐ b ‐PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene‐ b ‐poly(ethylene oxide) (PB‐ b ‐PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor‐dependent three‐enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.