On an Unstable Antifungal Metabolite from Trichoderma koningiiIsolation and Structure Elucidation of a New Cyclopentenone Derivative(3-Dimethylamino-S-hydroxy-5-vinyl-2-cyclopenten-1-one).

Abstract

In the course of our search for new antifungal antibiotic from fungi we had isolated a fungal strain Y-87,2100 which produced a metabolite active against Candida albicans. The bioactive compoundwas unstable, losing its activity during concentration of its solution and had to be converted to a stable molecule 2 for characterisation. In this paper we report the fermentation and isolation of 1 as well as conversion of 1 to 2 followed by structure elucidation of 2. The fungal strain Y-87,2100, isolated1* from a piece of rotten wood sample collected in Mulund, Bombay, was identified as Trichoderma koningii Oudemans. The strain was maintained either on potato dextrose agar or Sabouraud's dextrose agar. The strain has been deposited as reference with the GermanCollection of Microorganisms and Cell Cultures, Braunschweig,