Correlation between<i>Helicobacter pylori</i>OipA Protein Expression and<i>oipA</i>Gene Switch Status

Abstract

Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.