Journal of Clinical Microbiology · 1988 · 37 citations · 17 references
Life SciencesImmunologyInfectious DiseasesClinical ImmunologyImmunology Of Infectious DiseasesImmunotechnologyAntibody ScreeningEnzyme-linked Immunosorbent AssayHiv DiagnosisHiv TestingImmunoassay DevelopmentImmunodeficiency DisordersAcquired Immunodeficiency SyndromeCompetitive Enzyme-linked Immunosorbent AssayEnzyme ImmunoassaysEnzyme-linked Immunosorbent Assay (Elisa)Hiv Antibody DetectionHiv Serologic TestingImmunoassaysWestern Blot Analysis
The efficacies of nine enzyme-linked immunosorbent assays (EIA) for antibody to human immunodeficiency virus type 1 (HIV-1) and one EIA for antibody to HIV-2 in detecting antibody to HIV-2 were studied. The competitive EIAs for antibody to HIV-1 were less sensitive than the indirect EIAs. The overall prevalence of positive results was between 28 and 51% with the competitive EIAs and between 70 and 93% with the indirect EIAs. Most of the EIAs were less sensitive in detecting antibody to HIV-2 in sera from people with acquired immunodeficiency syndrome-like diseases than in sera from symptomless individuals. The results indicate that there is a high degree of cross-reactivity between HIV-1 and HIV-2 by EIA, indicating that serotype specificity must be determined by Western blot (immunoblot) with both sets of viral antigens. The results are relevant for discussing public health strategies, especially the screening of blood donors; competitive EIAs for antibody to HIV-1 are not sensitive enough to be used in areas where HIV-2 is prevalent (West Africa).
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Isolation of a New Human Retrovirus from West African Patients with AIDS
François Clavel, Denise Guétard, Françoise Brun‐Vézinet et al. · Science · 1986 · 1.3K citations
Genome organization and transactivation of the human immunodeficiency virus type 2
Mireille Guyader, Michael Emerman, P. Sonigo et al. · Nature · 1987 · 837 citations