No Age-Related Change in Circulating Endothelial Progenitor Cells in Healthy Subjects

Abstract

Endothelial progenitor cells were isolated from peripheral blood obtained from 32 healthy volunteers without cardiovascular risk factors who ranged in age from 20 to 61 years (mean [+/- SD] age, 34.1 +/- 9.6 years). The fractions of CD34(+) endothelial progenitor cells expressing kinase insert domain receptor-1, CD62E, or CD31 were analyzed with flow cytometry. Correlation analysis demonstrated that there was no significant correlation between subject age and the fraction of circulating CD34(+) mononuclear cells expressing kinase insert domain receptor-1 (P = 0.324; r = -0.180). Similarly, there was no significant correlation between subject age and the fraction of circulating CD34(+) mononuclear cells expressing CD62E (P = 0.496; r = -0.125) or the fraction of circulating CD34(+) mononuclear cells expressing CD31 (P = 0.245; r = -0.212). In conclusion, the experimental results showed that there was no age-related change in the basal level of circulating endothelial progenitor cells in healthy subjects without cardiovascular risk factors.