Sporulation in Bacillus subtilis. Genetic Analysis of Oligosporogenous Mutants

Abstract

SUMMARY Mutations in 30 Oligosporogenous (Osp) mutants of Bacillus subtilis have been mapped by transduction with phage PBS-1. The sporulation phenotype of these mutants had previously been characterized biochemically and morphologically. The mutationsare situated in areas of the genome previously shown to be occupied by asporogenous (Sp-) mutations. Data from reciprocal transformation between Ospand Sp- mutations of indistinguishable phenotype suggest that one of the Osp mutations and at least one of the Sp- mutations may be located within the same gene.