Effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (bubalus bubalis) spermatozoa.

Abstract

Equilibration time is necessary for preservation of the motility and integrity of buffalo sperm membranes. Equilibration times of over than 2 hours resulted in the greatest preservation of total semen parameters during cryopreservation. There were no significant interactions between equilibration times over 4 hours and type of extender which lead to greater post thaw sperm survival.