Inducing and Sensing Protein–Protein Interactions in Living Cells by Selective Cross‐linking

Abstract

Cells technique: Small molecules have been synthesized that enable the covalent and irreversible dimerization of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT or SNAP-Tag) in vitro and in living cells. The cross-linking efficiency of AGT fusion proteins provides a measure to characterize the proximity and interactions of protein pairs in living cells (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z700408_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.