RADIOIMMUNOASSAY OF HUMAN GROWTH HORMONE USING THE DOUBLE ANTIBODY TECHNIQUE

Abstract

ABSTRACT The method of Roos et al. (1963) for the preparation of HGH yields a highly purified biologically active hormone preparation suitable for clinical purposes and, after one additional purification step (chromatography on Sephadex G-200 according to Hunter (1965), for radioiodination. In the double antibody technique described, the labelled hormone can be used for radio-immunoassay without starch-gel or Sephadex purification. The method is simple and sensitive enough to permit the determination of HGH in serum.