Science · 2015 · 619 citations · 28 references
Life SciencesBiotechnologyGeneticsGenetic EngineeringGenome EditingVirologyAnimal VirusesCrispr-cas SystemsSwine GenomicsSwine VirologyTransgenic Animal TechnologyXenotransplantationEndogenous Retrovirus InactivationGene Editing In PigsGenome ReplicationRetroviral Gene TransferRetroviral VectorsRetrovirus BiologyTransgenic Pigs
Pig transplants could address organ shortages, but endogenous retroviruses pose a risk of cross‑species infection. The authors integrated CRISPR‑Cas into pig cells, continuously expressing Cas9 to mutate every PERV reverse‑transcriptase gene. This strategy eliminated replication of all PERV copies, preventing viral infection and transmission to human cells. Yang et al., Science, p.1101.
Virally cleansing the pig genome Transplants from pigs could be a solution to a shortage of human organs for transplantation. Unfortunately, porcine endogenous retroviruses (PERVs) are rife in pigs and can be transmitted to humans, risking disease. L. Yang et al. integrated CRISPR-Cas into the pig cell genome, where continuous induction of the Cas9 editing enzyme resulted in the mutation of every single PERV reverse transcriptase gene. This prevented replication of all copies of PERV, viral infection, and transmission to human cells. Science , this issue p. 1101
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