Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance

Abstract

Abstract The inheritance of resistance to lentil ( Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp. lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692–16‐l(s)) lines. F 2:4 progenies and F 6:8 , F 6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well‐established wilt‐sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified for the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK‐15 900 ) at 10.8 cM. Two other RAPD markers (OP‐BH 800 and OP‐DI5 500 ) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP‐C04 650 ) was associated with repulsion. The DNA markers reported here will provide a starting point in marker‐assisted selection for vascular wilt resistance in lentil.