<i>In vivo</i> and <i>in vitro</i> binding of carbon tetrachloride with nucleic acids and proteins in rat and mouse liver

Abstract

Abstract 14 C‐labelled carbon tetrachloride binds in vivo to DNA of mouse liver and to r RNA of rat liver if the animals have been pretreated with 3‐methylcholanthrene. A noticeable amount of radioactivity is also observed in liver proteins. In vitro carbon tetrachloride is activated by microsomes and pH 5 enzymes of 3‐methylcholanthrene‐treated animals to a metabolite which can react with DNA and polynucleotides; this effect is more evident if subcellular fractions from mouse liver are used.