Inhibitory Effect of Ultraviolet-inactivated Vesicular Stomatitis Virus on Initiation of DNA Synthesis in Cultured Chick Embryo Cells

Abstract

SUMMARY The initiation of partially synchronized DNA synthesis was induced by medium replacement on stationary monolayer cultures of chick embryo cells. The synthesis of DNA started at 4 hr and continued for 8 hr, and cells divided within 15 to 18 hr. The onset of DNA synthesis and subsequent cell division were inhibited by u.v.-inactivated vesicular stomatitis virus if the cells were infected before the onset of DNA synthesis. In contrast, if the cells were infected during the S phase, the continuing DNA replication and subsequent cell division were not inhibited. Thus the selective inhibition of the flow of G1 cells to the S phase was demonstrated in the cells infected with u.v.-inactivated vesicular stomatitis virus.