The Double Edge of Reactive Oxygen Species as Damaging and Signaling Molecules in HL60 Cell Culture

Abstract

HL60 cells respond to exposure to sustained levels of H(2)O(2) in a dose-response manner to H(2)O(2) concentration by activating the expression and activity of the antioxidant machinery, although the activation of the antioxidant defenses is not enough to avoid the appearance of oxidative damage. Of the two designs proposed, continuous exposure seems to be more appropriate to study the antioxidant response of HL60 cells to H(2)O(2).