Standardization of the Processing of Human Bone Marrow for Allogeneic Transplantation

Abstract

Allogeneic bone marrow transplantation has been undertaken within this centre on 62 patients with acute or chronic leukaemia. Employing the standard separation protocol described, all bone marrows were processed on the 2991 Blood Cell Processor to isolate the 'buffy coat' cells and subsequently the mononuclear cell component using a density separation medium of Ficoll-metrizoate. Following the mononuclear cell separation, the cells were identified for their T lymphocyte component using a combination of two murine monoclonal antibodies, MBG6 reacting with a pan T cell antigen (CD6) and RFT8 detecting the 'cytotoxic/supressor' cell antigen (CD8). The numerical results for nucleated cells, red blood cells, T lymphocytes and colony forming units-granulocyte macrophage are presented.