Flexibility of Melting Temperature Assay for Rapid Detection of Insertions, Deletions, and Single-Point Mutations of the AGXT Gene Responsible for Type 1 Primary Hyperoxaluria

Abstract

Nine PH1 patients, whose mutations had first been analyzed by the single-strand conformation polymorphism (SSCP) technique and then by sequencing of abnormal mobility bands of four AGXT exons (5), were studied comparatively by the melting temperature assay (MTA).