Encoded Fiber‐Optic Microsphere Arrays for Probing Protein–Carbohydrate Interactions

Abstract

Protein–carbohydrate interactions can be evaluated by using randomly ordered fiber optic microsphere arrays bearing immobilized synthetic oligosaccharides (see scheme). Two systems are examined: the mannose-binding lectin concanavalin A, and cyanovirin N. The latter allows the simultaneous evaluation of five distinct structures against a carbohydrate-binding protein with unambiguous results.