Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts

TLDR

Microgravity induces bone loss in humans by reducing osteoblast‑mediated bone formation. The study tests whether simulated microgravity alters osteoblast gene expression, contributing to bone loss. Researchers used a Random Positioning Machine to expose 2T3 preosteoblasts to simulated microgravity, then performed DNA microarray and validated key gene changes with RT‑PCR and immunoblotting. Simulated microgravity for up to 9 days suppressed alkaline phosphatase activity without affecting proliferation, altered expression of 140 genes (88 down, 52 up), including downregulation of ALP, Runx2, osteomodulin, PTHR1, upregulation of cathepsin K, and unchanged BMP4 and cystatin C, highlighting gravisensitive genes relevant to bone loss and osteoporosis.

Abstract

Exposure to microgravity causes bone loss in humans, and the underlying mechanism is thought to be at least partially due to a decrease in bone formation by osteoblasts. In the present study, we examined the hypothesis that microgravity changes osteoblast gene expression profiles, resulting in bone loss. For this study, we developed an in vitro system that simulates microgravity using the Random Positioning Machine (RPM) to study the effects of microgravity on 2T3 preosteoblast cells grown in gas-permeable culture disks. Exposure of 2T3 cells to simulated microgravity using the RPM for up to 9 days significantly inhibited alkaline phosphatase activity, recapitulating a bone loss response that occurs in real microgravity conditions without altering cell proliferation and shape. Next, we performed DNA microarray analysis to determine the gene expression profile of 2T3 cells exposed to 3 days of simulated microgravity. Among 10,000 genes examined using the microarray, 88 were downregulated and 52 were upregulated significantly more than twofold using simulated microgravity compared with the static 1-g condition. We then verified the microarray data for some of the genes relevant in bone biology using real-time PCR assays and immunoblotting. We confirmed that microgravity downregulated levels of alkaline phosphatase, runt-related transcription factor 2, osteomodulin, and parathyroid hormone receptor 1 mRNA; upregulated cathepsin K mRNA; and did not significantly affect bone morphogenic protein 4 and cystatin C protein levels. The identification of gravisensitive genes provides useful insight that may lead to further hypotheses regarding their roles in not only microgravity-induced bone loss but also the general patient population with similar pathological conditions, such as osteoporosis.