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Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples

Standard CurvesEnvironmental MonitoringEngineeringPassive SamplingAmplification EfficiencyCalibrationEnvironmental Analytical ChemistryBioanalysisMass SpectrometryNucleic Acid Amplification TestAnalytical ChemistryBiostatisticsGene QuantificationMicrobiologyNucleic Acid AmplificationEnvironmental AnalysisReal-time Polymerase Chain ReactionMedicine