GLYCEROLIPID BIOSYNTHESIS IN PEROXISOMES VIA THE ACYL DIHYDROXYACETONE PHOSPHATE PATHWAY*

Abstract

In recent years, acyl dihydroxyacetone phosphate [acyl-DHAPt) has been shown to be a precursor of glycerolipids and glycerol-ether lipids.'.' Acyl-DHAP was discovered as a rapidly labeled lipid that was formed in crude mitochondrial fraction from 3'Pi or y-32P [ATP].3This rapid labeling was due to the enzymatic dephosphorylation and rephosphorylation of endogenous acyl-DHAP present in the crude mitochondrial fraction (equation 1):Acyl-DHAP 2 -/ -Acyl-DHA(1) ADP ATPAcyl-DHAP was later shown to be biosynthesized by a direct acylation of DHAP with acyl-CoA.catalyzed