Limnobacter litoralis sp. nov., a thiosulfate-oxidizing, heterotrophic bacterium isolated from a volcanic deposit, and emended description of the genus Limnobacter

Abstract

A Gram-negative, aerobic, heterotrophic bacterium, designated KP1-19 T , was isolated from a 22-year-old volcanic deposit at a site lacking vegetation on the island of Miyake, Japan. Strain KP1-19 T was able to use thiosulfate (optimum concentration 10 mM) as an additional energy source. 16S rRNA gene sequence analysis indicated that strain KP1-19 T was closely related to Limnobacter thiooxidans CS-K2 T within the class Betaproteobacteria (97.7 % 16S rRNA gene sequence similarity). The cellular fatty acid profile was characteristic of the genus Limnobacter : the major fatty acids (>5 %) were C 16 : 0 , C 16 : 1 ω 7 c and C 18 : 1 ω 7 c and minor amounts of C 10 : 0 3-OH were also found. DNA–DNA relatedness between strain KP1-19 T and L. thiooxidans LMG 19593 T was 18 %. Therefore, strain KP1-19 T represents a novel species, for which the name Limnobacter litoralis sp. nov. is proposed. The type strain is KP1-19 T (=LMG 24869 T =NBRC 105857 T =CIP 109929 T ).