Polarization Transfer over the Water–Protein Interface in Solids

Abstract

A key to the elucidation of protein functions are water–protein interactions. The determination of polarization-transfer pathways between water and immobilized proteins demonstrates that chemical exchange (sometimes followed by spin diffusion) is the dominant mechanism. At low temperatures or high spinning frequencies, this pathway becomes inefficient, and intermolecular NOEs are observed (see NMR spectra; ROE=rotating frame NOE). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z801110_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.