Journal of Clinical Microbiology · 2002 · 67 citations · 26 references
Life SciencesInfectious DiseasesMicrobiologyGenomicsMolecular VirologyVirologyMolecular TypingViral ClassificationViral GeneticsViral GenomicsViral InfectionsVirus ClassificationGenetic Diversity Of VirusesGenomic Diversity AnalysisMultiplex Polymerase Chain Reaction (Pcr)Rna ElectrophoresisRotavirus GastroenteritisViral Diversity AssessmentViral Strain TypingVirus Genotypes
Between 1998 and 2000, a total of 266 samples were found positive for group A rotaviruses by RNA electrophoresis. Samples were collected from patients admitted to two leading hospitals in Calcutta. Serotyping could be done only with 22% of the positive samples, leaving 78% untypeable. The G (VP7 genotypes) and P (VP4 genotypes) types were determined for 159 samples by reverse transcription and multiplex PCR. The predominant genotype was G1P[8] (20%), followed by G2P[4] (15%) and G4P[8] (6%). A number of uncommon genotypes, G1P[4] (4%), G2P[8] (2.5%), G2P[6] (0.6%), G4P[4] (2.5%), and G4P[6] (1.25%), were also detected during this study period. Twenty two percent of specimens showed mixed infections, 38 (24%) of the total samples remained untypeable for either VP7 or VP4, while only 4 (2.5%) of the samples were untypeable for both genes. Eleven specimens collected from Manipur were also genotyped and revealed a very high degree of genomic reassortment.
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