Environmental and Molecular Mutagenesis · 2011 · 23 citations · 33 references
PharmacyCell BiologyToxicologyApoptosisEnvironmental ToxicologyMolecular ToxicologyDna Damage-induced ApoptosisGenotoxicity MechanismsHepatotoxicity MechanismsMechanisms Of Pesticide ToxicityMolecular Mechanisms Of ToxicityOrganophosphate ToxicologyAlkaline Comet AssayApoptosis-related Dna DamageDna Damage InductionDrug-induced Dna DamageGenotoxic Effects Of PesticidesOrganophosphorus PesticidesOxidative Dna DamagePesticide Genotoxicity
This study investigates the effects of fenthion and terbufos, two organophosphorous pesticides, on DNA damage, tumor-related gene expression, and apoptosis in HepG2 cells. We found that exposure to concentrations ranging from 50 to 200 μM of fenthion and terbufos for 2 hr caused significant death in HepG2 cells. Both compounds induced DNA damage in a concentration-dependent manner as measured using the alkaline comet assay. Tumor-related genes (jun, myc, and fos) and apoptosis-related genes (socs3, tnfaip3, ppp1r15a, and nr4a1) were up-regulated by both compounds. Finally, both compounds induced apoptosis. The results demonstrate that both terbufos and fenthion induce DNA damage and should be considered potentially hazardous to humans.
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Statistical analysis of real-time PCR data
Joshua S. Yuan, Ann M. Reed, Feng Chen et al. · BMC Bioinformatics · 2006 · 2.2K citations · Full text
jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun
J. Schütte, Jean Viallet, Marion M. Nau et al. · Cell · 1989 · 487 citations · Full text