Arcicella aurantiaca sp. nov., isolated from stream water

Abstract

A bacterial strain designated TNR-18 T was isolated from stream water in southern Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TNR-18 T were Gram-stain-negative, strictly aerobic, non-motile and vibrioid, and the strain formed orange colonies. Growth occurred at 10–30 °C, with 0–0.2 % NaCl and at pH 6.0–10.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-18 T belonged to the genus Arcicella ; its closest neighbours were Arcicella rosea TW5 T and Arcicella aquatica NO-502 T , with respective sequence similarities of 97.9 and 97.8 %. Predominant cellular fatty acids (>10 %) were summed feature 3 (C 16 : 1 ω 7 c /C 16 : 1 ω 6 c ; 37.7 %), C 16 : 1 ω 5 c (13.0 %) and iso-C 15 : 0 (11.1 %). The major respiratory quinone was MK-7. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized polar lipids. The DNA G+C content was 39.9 mol%. On the basis of the phylogenetic and phenotypic data, strain TNR-18 T should be classified as representing a novel species, for which the name Arcicella aurantiaca sp. nov. is proposed. The type strain is TNR-18 T (=LMG 25207 T =BCRC 17969 T ).