Journal of General Virology · 1987 · 18 citations · 8 references
Life SciencesGeneticsMolecular GeneticsGenomicsMolecular VirologyVirologyViral Genome OrganizationViral GenomicsViral StructureVirus Genome OrganizationVirus StructureYellow Fever17D VaccineCoding Region VariationViral Genome StructureViral Structural GenomicsYellow Fever VaccinationYellow Fever Virus
The genome of the Pasteur 17D-204 vaccine strain of yellow fever virus has been cloned into pBR327. The inserts of recombinant plasmids were analysed by restriction cleavage pattern and compared with that of the genome of another substrain previously cloned and sequenced. Ten of the overlapping inserts were found to contain the sequence of the complete genome. We have sequenced approximately 3680 bases of the 5' region which codes for the C, M and E structural proteins and the NS1 non-structural protein. This sequence is the same as that reported previously, indicating a remarkable stability of these two vaccine substrains.
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A simple and very efficient method for generating cDNA libraries
Ueli Gubler, Beth J. Hoffman · Gene · 1983 · 4.6K citations
High-Efficiency Cloning of Full-Length cDNA
Hiroto Okayama, Paul Berg · Molecular and Cellular Biology · 1982 · 1.3K citations · Full text