DNA probe detection of periodontal pathogens

Abstract

The technology for hybridization of nucleic acids using isotopically labeled whole genomic or species‐specific cloned deoxyribonucleic acid (DNA) probes for Actinobacillus actinomycetemcomitans, Bacteroides intermedius and Bacleroides gingivulis can be used to detect as few as 102 cells of these periodontal pathogens. The sensitivity and specificity of the test is not affected by the presence of 5x 10s unrelated bacteria in constructed mixed culture samples. Current feasibility tests with non‐isotopic reagents also give reliable and rapid detection of these oral pathogens in pure or mixed cultures. DNA probes should prove useful in a rapid, specific, and sensitive assay for these periodontal pathogens.