Allopolyploidy-Induced Rapid Genome Evolution in the Wheat (<i>Aegilops</i>–<i>Triticum</i>) Group

TLDR

The sequences examined are present in all diploid Triticeae species but are confined to single genomes in polyploid wheats, existing as chromosome‑specific or genome‑specific repeats. The study aimed to determine the rate and timing of elimination of eight DNA sequences during allopolyploid formation in Aegilops–Triticum hybrids. The authors analyzed 35 F1 hybrids and 22 derived allopolyploids, comparing them with parental plants to assess sequence elimination. Rapid, nonrandom elimination of chromosome‑specific and genome‑specific sequences occurs early in allopolyploid development, is governed by genomic composition rather than parental genotype or ploidy, and likely facilitates homoeologous chromosome differentiation and the successful establishment of new polyploid species.

Abstract

To better understand genetic events that accompany allopolyploid formation, we studied the rate and time of elimination of eight DNA sequences in F1 hybrids and newly formed allopolyploids of Aegilops and TRITICUM: In total, 35 interspecific and intergeneric F1 hybrids and 22 derived allopolyploids were analyzed and compared with their direct parental plants. The studied sequences exist in all the diploid species of the Triticeae but occur in only one genome, either in one homologous pair (chromosome-specific sequences [CSSs]) or in several pairs of the same genome (genome-specific sequences [GSSs]), in the polyploid wheats. It was found that rapid elimination of CSSs and GSSs is a general phenomenon in newly synthesized allopolyploids. Elimination of GSSs was already initiated in F1 plants and was completed in the second or third allopolyploid generation, whereas elimination of CSSs started in the first allopolyploid generation and was completed in the second or third generation. Sequence elimination started earlier in allopolyploids whose genome constitution was analogous to natural polyploids compared with allopolyploids that do not occur in nature. Elimination is a nonrandom and reproducible event whose direction was determined by the genomic combination of the hybrid or the allopolyploid. It was not affected by the genotype of the parental plants, by their cytoplasm, or by the ploidy level, and it did not result from intergenomic recombination. Allopolyploidy-induced sequence elimination occurred in a sizable fraction of the genome and in sequences that were apparently noncoding. This finding suggests a role in augmenting the differentiation of homoeologous chromosomes at the polyploid level, thereby providing the physical basis for the diploid-like meiotic behavior of newly formed allopolyploids. In our view, this rapid genome adjustment may have contributed to the successful establishment of newly formed allopolyploids as new species.