Study of genetic variability of Wormwood capillary (Artemisia capillaris) using inter simple sequence repeat (ISSR) in Pahang region, Malaysia.

Abstract

The genetic variability of five individuals of Artemisia capillaris from state of Pahang, Malaysia was examined using Inter Simple Sequence Repeat (ISSR) technique. Samples were collected from different regions in Pahang State. Results showed distinctive ISSR banding pattern in this species. Twenty five primers were applied and only ten were selected (807, 809, 825, 834, 841, 862, 866, 876, nIssr 1 and nIssr 3) as reliable amplifying ISSR markers. A total of 90 ISSR bands including 62 polymorphic (68.89%) with amplicon size ranging from 150– 2500 bp were scored. Genetic distance for samples ranged from 0.0500 to 0.4200. For similarity index samples were ranged from 0.6400 to 0.9545.