Metabolism of Pipecolic Acid in a <i>Pseudomonas</i> Species V. Pipecolate Oxidase and Dehydrogenase

Abstract

Oxidation of pipecolate to Delta(1)-piperideine-6-carboxylate is catalyzed by pipecolate oxidase, an inducible, membrane-bound dehydrogenase associated with the electron transport components of Pseudomonas putida P2. From the oxidase, we obtained a smaller particle containing flavine adenine dinucleotide (FAD) and cytochrome b, but no longer able to catalyze electron transfer to oxygen or to cytochrome c. Certain properties of this l-pipecolate dehydrogenase, an FAD-flavoprotein, are reported.