Pronounced Effect of DNA Hybridization on Click Reaction Efficiency

Abstract

Abstract We report about the efficiency of the click reaction directly on PCR products containing alkyne‐modified uridine nucleosides. Galactose azide was clicked onto single‐stranded and double‐stranded 300mer DNA in order to investigate the efficiency of sugar labeling of DNA under various conditions. Single‐stranded alkyne‐modified DNA was prepared by selective lambda exonuclease digestion of 5′‐phosphate‐labeled double strands. A comparison of the click reaction efficiency on modified single and double stranded 300mers under identical conditions shows a pronounced effect of the strandedness on the yield of the click reaction.