Isolation and Characterization of Constituents from the Leaves of Xanthium strumarium and their Evaluation for Antioxidant and Antimicrobial Potential

Abstract

IR spectra was recorded (KBr disc) on FT-IR spectrometer; Bruker ADVANCE II 400 NMR spectrophotometer was used to record 1 H-NMR chemical shifts () are reported as downfield displacements from TMS used as internal standard and coupling constants are reported in Hz; WATERS Q-T OF MICROMASS (LC-MS) was used to record mass spectrum of compounds from SAIF, Punjab University, Chandigarh. SHIMAZDU UV -1800 spectrophotometer was used for UV analysis. Electronic balance (CY 200) of Denver instruments was used for weighing. TLC was performed with precoated aluminium silica gel GF 254. All solvents were analytical grade. Isolated phytoconstituents were tested by performing chemical test such as Libermann-buchard's reagent, Salkowski test, Spot test, Litmus paper test etc and determining the physical parameters like appearance, solubility and melting point by comparing with literature.