Concepedia

A New Micromethod for Lymphocyte Stimulation Using Whole Blood

J. Eskola, Esa Soppi, M Viljanen, Olli Ruuskanen

Immunological Communications · 1975 · 97 citations · 12 references

DOI

Concepts

Abstract

A new micromethod for stimulation of human lymphocytes is described. The use of small amounts of whole blood, microtiter plates, 125I-5-iodo-2'-deoxyuridine, 5-fluoro-2'-deoxyuridine and a multiple cell-culture harvester makes the method easy, rapid, reproducible and suitable for routine laboratory use.

References

12

Whole-blood culture technique for functional studies of lymphocyte reactivity to mitogens, antigens, and homologous lymphocytes.

John L. Pauly, Joseph E. Sokal, Tin Han · PubMed · 1973 · 126 citations

, , +11

Microtechnique for quantitative evaluation of in vitro lymphocyte transformation.

Ulrich Junge, J. Hoekstra, Lauren G. Wolfe et al. · PubMed · 1970 · 100 citations

, , +14

A New Micromethod for Evaluating Lymphocyte Responses to Phytohemagglutinin: Quantitative Analysis of the Function of Thymus-Dependent Cells

B. H. Park, Robert A. Good · Proceedings of the National Academy of Sciences · 1972 · 96 citations

, , +10

Simplified whole blood method for evaluating in vitro lymphocyte reactivity of laboratory animals.

Tao Han, John L. Pauly · PubMed · 1972 · 68 citations

, , +12

Selective Activation of Chicken T Lymphocytes by Concanavalin A

Paavo Toivanen, Auli Toivanen · The Journal of Immunology · 1973 · 59 citations

, , +13