American Journal of Botany · 2011 · 19 citations · 6 references
Life SciencesGeneticsPlant SciencesMolecular BreedingPlant GeneticsPlant GenomicsGenetic Diversity AssessmentMolecular Markers In PlantsPopulation Genetics In PlantsGenomic Dna Library ConstructionGenomic Library ConstructionMicrosatellite AnalysisMicrosatellite Marker AnalysisMicrosatellite Marker DevelopmentMicrosatellite MarkersMicrosatellite VariationPopulation Genetic Structure
PREMISE OF THE STUDY: Microsatellite primers were developed in Paeonia lactiflora for further population genetic studies. • METHODS AND RESULTS: Ten dinucleotide microsatellite markers were developed using an enriched genomic library. They were identified in 58 individuals, and nine markers were also amplified in its cultivars. The number of alleles per locus ranged from three to 11, with a mean of six. The observed and expected heterozygosities ranged from 0.1662 to 0.9140 and from 0.0841 to 0.8157, respectively. • CONCLUSIONS: These microsatellite markers will facilitate further studies on population genetics variation and genetic structure of P. lactiflora.
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A rapid DNA isolation procedure for small amounts of fresh leaf tissue
JJ Doyle, JL Doyle, J.J. Doyle et al. · Medical Entomology and Zoology · 1987 · 2.4K citations