Non‐covalent Single Transcription Factor Encapsulation Inside a DNA Cage

Abstract

CAP-turing the moment: A cage made from DNA can be used to encapsulate a transcription factor (catabolite activator protein, CAP; see figure) without covalent attachment. CAP is shown to stably bind inside the DNA cage at a 1:1 ratio by bending one edge to accommodate the protein. Single-molecule fluorescence measurements confirm the orientation of CAP within the cage. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.